Heroes[]
- Spider-Man (Voiced by Neil Patrick Harris)
- War Machine (Voiced by Dorian Harewood)
- Nightcrawler (Voiced by Nolan North)
- Daredevil (Voiced by Brian Bloom)
- Mr. Fantastic (Voiced by Cam Clarke)
- Iron Man (Voiced by Adrian Pasdar)
- Hawkeye (Voiced by Thomas F. Wilson)
- Black Panther (Voiced by Tim Russ)
- Spider-Woman (Voiced by Tasia Valenza)
- Ka-Zar (Voiced by John Cygan)
- The Wasp (Voiced by Grey DeLisle)
- Cyclops (Voiced by Robin Atkin Downes)
- Jean Grey (Voiced by Leigh Allyn-Baker)
- The Beast (Voiced by Kevin Michael Richardson)
- Scarlet Witch (Voiced by Terri Hawkes)
- Wonder Man (Voiced by Steven Blum)
- Iron Fist (Voiced by Peter Dobson)
- Moon Knight (Voiced by Robin Atkin Downes)
- Silver Surfer (Voiced by Chris Cox)
- Invisble Woman (Voiced by Danica McKellar)
- She Hulk (Voiced by Maria Canals-Barrera)
- Falcon (Voiced by Khary Payton)
- Punisher (Voiced by Daran Norris)
- Psylocke (Voiced by Grey DeLisle)
- Songbird (Voiced by Susan Spano)
- Thor (Voiced by Rick D. Wasserman)
- Quicksilver (Voiced by Mark Hildreth)
- Polaris (Voiced by Terri Hawkes)
- Prowler (Voiced by Tim Russ)
- Longshot (Voiced by James Arnold Taylor)
- Namor (Voiced by Joe J. Thomas)
- Namorita (Voiced by Jodi Benson)
- Elektra (Voiced by Gabrille Carteris)
- Hercules (Voiced by John DiMaggio)
- Luke Cage (Voiced by Greg Eagles)
- Mockingbird (Voiced by Elizabeth Daliy)
- Emma Frost (Voiced by Kari Wahlgren)
- Vision (Voiced by Roger Rose)
- Black Knight (Voiced by J. B. Blanc)
- Ant-Man (Voiced by Wally Wingert)
- Doc Samson (Voiced by Cam Clarke)
- Nova (Voiced by Robert Tinkler)
- Black Widow (Voiced by Vanessa Marshall)
- Ms. Marvel (Voiced by April Stewart)
- Jubilee (Voiced by Danica McKellar)
- Professor Xavier (Voiced by Richard McGonagle)
- Rogue (Voiced by Erin Matthews)
- Colossus (Voiced by Tim Russ)
- Gambit (Voiced by J. B. Blanc)
- Sunfire (Voiced by James Sie)
- Shang-Chi (Voiced by James Sie)
- Tigra (Voiced by Tasia Valenza)
- Shanna The She Devil (Voiced by Masasa Moyo)
- Adam Warlock (Voiced by Jim Cummings)
- Bishop (Voiced by Khary Payton)
- Cable (Voiced by Lawerence Bayne)
- Angel/Archangel (Voiced by Dave Wittenberg)
- Goliath (Voiced by Gary Anthony Williams)
- Captain Mar-Vell (Voiced by Roger Rose)
- Havok (Voiced by Josh Keaton)
- Arachne (Voiced by Terri Hawkes)
- Satana (Voiced by Kim Mai Guest)
- Howard The Duck (Voiced by Jeff Bergman)
- Thundra (Voiced by Suasn Eisenberg)
- Hellcat (Voiced by Gina Torres)
- Misty Knight (Voiced by Susan Dailan)
- Banshee (Voiced by Quinton Flynn)
- Cloak (Voiced by Ahmed Best)
- Dagger (Voiced by Tara Strong)
- X-23 (Voiced by Tara Strong)
- Forge (Voiced by Lou Diamond Phillips)
- US Agent (Voiced by Steven Blum)
- Captain Britain (Voiced by Brian Bloom)
- Firebird (Voiced by Grey DeLisle)
- Beta Ray Bill (Voiced by Jim Cummings)
- Blink (Voiced by Tara Strong)
- Black Cat 2099 (Voiced by Katee Sackhoff)
- Maria Hill (Voiced by Margret Easley)
- Dum Dum Dugan (Voiced by John DiMaggio)
- Clay Quartermain (Voiced by Diedrich Bader)
- Jimmy Woo (Voiced by James Sie)
- Jasper Sitwell (Voiced by Cam Clarke)
- Valentina Allegra de la Fontaine (Voiced by Grey DeLisle)
- Phil Coulson (Voiced by Clark Gregg)
- Werewolf by Night (Voiced by David Sobolov)
- Sunpyre (Voiced by Kim Mai Guest)
- Magik (Voiced by Tara Strong)
- Ultimate Nick Fury (Voiced by Dave Fennoy)
- Magik (Voiced by Tara Strong)
- Odin (Voiced by Clancy Brown)
- Heimdall (Voiced by J. B. Blanc)
- Tyr (Voiced by Steven Blum)
- Sif (Voiced by Grey DeLisle)
- Hermond (Voiced by Josh Keaton)
- Balder (Voiced by Nolan North)
- Valkyrie (Voiced by Kari Wahlgren)
- Volstagg (Voiced by Fred Tataisciore)
- Hogun (Voiced by John DiMaggio)
- Fandral (Voiced by Chris Cox)
- Black Bolt (No Voice Actor)
- Medusa (Voiced by Iona Morris)
- Crystal (Voiced by Kim Mai Guest)
- Triton (Voiced by Mark Hamill)
- Gorgon (Voiced by Greg Eagles)
- Karnak (Voiced by Philip Proctor)
- Lockjaw (No Voice Actor)
- Guardian (Voiced by Jim Ward)
- Sasquatch (Voiced by Clancy Brown)
- Vindicator (Voiced by Tricia Helfer)
- Puck (Voiced by Dana Snyder)
- Snowbird (Voiced by Terri Hawkes)
- Northstar (Voiced by Rino Romano)
- Aurora (Voiced by Grey DeLisle)
- Shaman (Voiced by Mark Hamill)
- Noir Wolverine (Voiced by Steven Blum)
- Captain America (Voiced by Brian Bloom)
- Col. Nick Fury (Voiced by Jim Cummings)
- Black Cat (Voiced by Tricia Helfer)
- Dr. Strange (Voiced by James Horan)
- Speedbal/Penance (Voiced by Benjamin Diskin)
- Shadowcat (Voiced by Kim Mai Guest)
- Lockheed (No Voice Actor)
- Ghost Rider (Voiced by Nolan North)
- Wolverine (Voiced by Diedrich Bader)
- The Hulk (Voiced by Peter Lurie)
- Blade (Voiced by Khary Payton)
- The Thing (Voiced by Clancy Brown)
- Deadpool (Voiced by John Kassir)
- Bob, Agent of HYDRA (Voiced by Steven Blum)
- Weasel (Voiced by Cam Clarke)
- Storm (Voiced by Dawnn Lewis)
- Human Torch (Voiced by Quinton Flynn)
- Iceman (Voiced by James Arnold Taylor)
- Firestar (Voiced by Andrea Baker)
- Tony Stark (Voiced by John Cygan)
- Madame Web (Voiced by Susanne Blakeslee)
Spider-Men:[]
- Ultimate Spider-Man (Voiced by Drake Bell)
- 2099 Spider-Man (Voiced by Quinton Flynn)
- Noir Spider-Man (Voiced by Chirstopher Daniel Barnes)
- 1602 Spider-Man (Voiced by Josh Keaton)
- Scarlet Spider (Voiced by Rino Romano)
- Spider-Ham (Voiced by Benjamin Diskin)
Guest Allies:[]
- Uatu The Watcher (Voiced by Leonard Nimoy)
- Edwin Jarvis (Voiced by Philip Proctor)
- Wong (Voiced by George Takei)
- Franklin Richards (Voiced by Logan Grove)
- H.E.R.B.I.E. (Voiced by Cam Clarke)
- Wyatt Wingfoot (Voiced by Dave Wittenberg)
- Zeus (Voiced by Fred Tataisciore)
- Dr. Jane Foster (Voiced by Kari Wahlgren)
- Bruce Banner (Voiced by Neal McDonough)
- J. Jonah Jameson (Voiced by Daran Norris)
- Betty Brant (Voiced by Grey DeLisle)
- Robbie Robertson (Voiced by Kevin Michael Richardson)
- Ned Leeds (Voiced by Quinton Flynn)
- Ben Parker (Voiced by Edward Asner)
- May Parker (Voiced by Marion Ross)
- Richard Parker (Voiced by Jim Cummings)
- Mary Parker (Voiced by Kath Souice)
- Norman Osborn (Voiced by Steven Weber)
- Harry Osborn (Voiced by Benjamin Diskin)
- Mary Jane Watson (Voiced by Vaneesa Marshall)
- Liz Allen (Voiced by Grey DeLisle)
- Gwen Stacy (Voiced by Lacey Chabert)
- Cap. George Stacy (Voiced by Clancy Brown)
Villians[]
- Doctor Doom (Voiced by Clive Revill)
- Galactus (Voiced by Clancy Brown)
- Batroc The Leaper (Voiced by J. B. Blanc)
- Red Skull (Voiced by Clancy Brown)
- Loki (Voiced by Larry Cedar)
- Enchantress (Voiced by Gabirelle Carteris)
- Execuntioner (Voiced by Peter Lurie)
- Baron Mordo (Voiced by Philip Proctor)
- M.O.D.O.K. (Voiced by Wally Wingert)
- Scientist Supreme (Voiced by Nolan North)
- A.I.M. Troopers (Voiced by Steven Blum)
- Professor Thornton (Voiced by Mark Hamill)
- Sabretooth (Voiced by Peter Lurie)
- Lady Deathstrike (Voiced by Kim Mai Guest)
- Omega Red (Voiced by Mark Hamill)
- Thunderbolt Ross (Voiced by Keith Ferguson)
- Rhino (Voiced by Peter Lurie)
- Attuma (Voiced by Gregg Berger)
- Andromeda (Voiced by Jodi Benson)
- Tiger Shark (Voiced by Beau Weaver)
- Warlord Krang (Voiced by Fred Tataisciore)
- Byrrah (Voiced by James Horan)
- Orka (Voiced by Dwight Schultz)
- Llyra (Voiced by Tricia Helfer)
- Magneto (Voiced by Clancy Brown)
- Mystique (Voiced by Grey DeLisle)
- Pyro (Voiced by John Kassir)
- Blob (Voiced by Peter Lurie)
- Toad (Voiced by Armin Shimmerman)
- Avalanche (Voiced by Peter Lurie)
- Mastermind (Voiced by John DiMaggio)
- Multiple-Man (Voiced by Wally Wingert)
- Juggernaut (Voiced by John DiMaggio)
- Egghead (Voiced by John DiMaggio)
- Kraven The Hunter (Voiced by Jim Cummings)
- Klaw (Voiced by Mark Hamill)
- Thanos (Voiced by Gary Anthony Williams)
- Dormmamu (Voiced by Michael T. Wiess)
- Impossible Man (Voiced by Dana Snyder)
- Baron Strucker (Voiced by Jim Ward)
- Grim Reaper (Voiced by Lance Henriksen)
- Wrecking Crew
- Wrecker (Voiced by J. B. Blanc)
- Piledriver (Voiced by Nolan North)
- Bulldozer (Voiced by Diedrich Bader)
- Thunderball (Voiced by Gary Anthony Williams)
- Madame Hydra (Voiced by Vanessa Marshall)
- Venom (Voiced by Quinton Flynn)
- Carnage (Voiced by Scott Cleverdon)
- Morbius (Voiced by Nolan North)
- Bastion (Voiced by Keith Szarabajka)
- Sentinels (Voiced by Jim Ward)
- Spilface Flanagan (Voiced by Brian Bloom)
- Joey Pulanski (Voiced by Keith Szarabajka)
- U-foes
- Vector (Voiced by Cam Clarke)
- X-Ray (Voiced by Keith Ferugson)
- Vapor (Voiced by Terri Hawkes)
- Iron Clad (Voiced by John DiMaggio)
- Absorbing Man (Voiced by Rick D. Wasserman)
- Shocker (Voiced by Diedrich Bader)
- Taskmaster (Voiced by Wade Williams)
- Kingpin (Voiced by David Sobolov)
- Hammerhead (Voiced by John DiMaggio)
- Jackal (Voiced by Brian George)
- Kang (Voiced by Jonathan Adams)
- Doctor Octopus 2099 (Voiced by Tara Strong)
- Scorpion 2099 (Voiced by John Kassir)
- Hobgoblin 2099 (Voiced by Mark Hamill)
- Mandarin (Voiced by James Sie)
- Whirlwind (Voiced by J. B. Blanc)
- Black Rose (Voiced by Rino Romano)
- Hypno Hustler (Voiced by Phil Lamarr) Vocals done by Neil Patrick Harris
- Inner Circle Club
- Sebastian Shaw (Voiced by Graham McTavish)
- Harry Leland (Voiced by Armin Shimmerman)
- Selene (Voiced by April Stewart)
- Donald Pierce (Voiced by Nolan North)
- Stepford Cuckoos (Voiced by Tara Strong, Kari Wahlgren, Terri Hawkes, Kim Mai Guest, Grey DeLisle)
- Ymir (Voiced by Gary Anthony Williams)
- Ulik (Voiced by'Fred Tataisciore)
- Kurse (Voiced by Tom Kane)
- Chemistro (Voiced by Gary Anthony Williams)
- Vulture (Voiced by Dwight Schultz)
- William Stryker (Voiced by Jim Cummings)
- Winter Soldier (Voiced by Rino Romano)
- Radioactive Man (Voiced by James Sie)
- Maximus The Mad (Voiced by Mark Hamill)
- Shadow King (Voiced by Dorian Harewood)
- The Skrulls
- Super Skrull (Voiced by Greg Eagles)
- Paibok (Voiced by Khary Payton)
- Titannus (Voiced by David Sobolov)
- Doctor Octopus (Voiced by Dave B. Mitchell)
- Mysterio (Voiced by Gregg Berger)
- Electro (Voiced by Thomas F. Wilson)
- Bullseye (Voiced by Peter Lurie)
- Abomination (Voiced by Robin Atkin Downes)
- Fin Fang Foom (Voiced by James Sie)
- Mole Man (Voiced by Gregg Berger)
- Mikahail Rasputain (Voiced by John DiMaggio)
- Dark Thunderbolts
- Baron Zemo (Voiced by Robin Atkin Downes)
- Moonstone (Voiced by Terri Hawkes)
- Green Goblin (Voiced by Armin Shimmerman)
- Beetle (Voiced by Daran Norris)
- Ghost (Voiced by Fred Tataisciore)
- Centurius (Voiced by Nolan North)
- Boomerang (Voiced by Steven Blum)
- Ultron (Voiced by James Horan)
- Mr. Sinister (Voiced by Don Leslie)
- Apocalpyse (Voiced by Richard McGonagle)
- Sauron (Voiced by John Kassir)
- Garrok (Voiced by Dwight Schultz)
- Arcade (Voiced by Quinton Flynn)
- Titanium Man (Voiced by Keith Ferguson)
- Stilt-Man (Voiced by Dorian Harewood)
- Mac Gargan (Voiced by Walter Bernet)
- Hobgoblin (Voiced by Mark Hamill)
- Dr. Walker Sloane (Voiced by Val Kilmer)
- Anti-Venom (Voiced by John Kassir)
- The Russian (Voiced by Nolan North)
- Jigsaw (Voiced by Richard McGonagle)
- Deacon Frost (Voiced by Steven Blum)
- Man-Ape (Voiced by Phil LaMarr)
- Shuma-Gorath (Voiced by John Kassir)
- Mephisto (Voiced by Fred Tataisciore)
- Blackheart (Voiced By David Sobolov)
- Strfye (Voiced by Lawerence Bayne)
- Dr. Bong (Voiced by Gregg Berger)
- The Tinkerer (Voiced by Philip Proctor)
- Noir Goblin (Voiced by Jim Cummings)